Cultrex 3D Culture Matrix Laminin I from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex 3D Culture Matrix Laminin I
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3446-005-01
Quantity30 mg
SpeciesMulti-Species
Molecule NameLaminin-1
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ApplicationsBioactivity
Format6 mg/ml
NCBI Full Gene Namelaminin subunit alpha 1
NCBI Gene AliasesLAMA, PTBHS, S-LAM-alpha
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium containing 10 µg/ml gentamycin sulfate and no phenol red. Sterility Testing: No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Hierarchical assembly of tryptophan zipper peptides into stress-relaxing bioactive hydrogelsNature communicationsOctober 23, 2023Nguyen AK, Molley TG, Kardia E, Ganda S, Chakraborty S, Wong SL, Ruan J, Yee BE, Mata J, Vijayan A, et al.

(2) PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systemsEMBO reportsAugust 3, 2023Kraus F, Goodall EA, Smith IR, Jiang Y, Paoli JC, Adolf F, Zhang J, Paulo JA, Schulman BA, Harper JW.,Thermo Fisher Scientific,17502048 Neurotrophin‐3,Peprotech,450‐03 Brain‐derived neurotrophic factor (BDNF),Peprotech,450‐02 Cultrex 3D Culture Matrix Laminin I,R&D Systems,3446‐005‐01 B27,Thermo Fisher Scientific,17504001 "B‐27 Supplement (50X), minus antioxidants",Thermo Fisher Scientific,10889038 Y‐27632

(3) Chemogenomic library design strategies for precision oncology, applied to phenotypic profiling of glioblastoma patient cellsiScienceJuly 21, 2023Paschalis Athanasiadis, Balaguru Ravikumar, Richard J.R. Elliott, John C. Dawson, Neil O. Carragher, Paul A. Clemons, Timothy Johanssen, Daniel Ebner, Tero Aittokallioproteins","Chemicals, peptides, and recombinant proteins" ,, Cultrex 3D matrix Laminin,R&D Systems rndsystems.com,3446-005-01 Hoechst-33342,MolProbes,H1399 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, TIBCO

(4) Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/β-catenin to induce exit from quiescenceCell reportsJune 27, 2023Robertson FL, O'Duibhir E, Gangoso E, Bressan RB, Bulstrode H, Marqués-Torrejón MÁ, Ferguson KM, Blin C, Grant V, Alfazema N, et al.

(5) Combinatorial selective ER-phagy remodels the ER during neurogenesisbioRxiv : the preprint server for biologyJune 26, 2023Hoyer MJ, Smith IR, Paoli JC, Jiang Y, Paulo JA, Harper JW.Dihydrochloride (ROCK inhibitor) PeproTech (1293823); Cultrex 3D Culture Matrix Laminin I R&D Systems (3446-005-01); Accutase StemCell (7920); FGF3 In-house (N/A); Insulin Human Sigma-Aldrich (I9278-5ML); TGF-beta PeproTech

(6) Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditionsJournal of Biological ChemistryMay 1, 2023Victoria Karakis, Mahe Jabeen, John W. Britt, Abigail Cordiner, Adam Mischler, Feng Li, Adriana San Miguel, Balaji M. RaoL-ascorbic acid,Sigma,Cat#A8960 Pen/Strep,Thermo Fisher Scientific,Cat#15140122 Laminin-111,R&D Systems,Cat#3446-005-01 Collagen IV,Corning,Cat#354233 Di-8-ANEPPS,Thermo Fisher Scientific,Cat#D3167 Pluronic acid F127,Thermo Fisher Scientific

(7) Embryonic Stem Cells Can Generate Oral Epithelia under Matrix InstructionInternational journal of molecular sciencesApril 22, 2023Das R, Harper L, Kitajima K, Osman TA, Cimpan MR, Johannssen AC, Suliman S, Mackenzie IC, Costea DE.

(8) A reference human induced pluripotent stem cell line for large-scale collaborative studiesCell stem cellDecember 1, 2022Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, et al.supplement,Thermo Fisher Scientific,17504044 BDNF,PeproTech,450-02 NT3,PeproTech,450-03 Laminin,R&D Systems,3446-005-01 Compound E,Stem Cell Technologies,73952 poly-L-ornithine (PLO),Sigma-Aldrich,A-004-M Culture One

(9) Neuronal-epithelial cross-talk drives acinar specification via NRG1-ERBB3-mTORC2 signalingDevelopmental cellNovember 21, 2022May AJ, Mattingly AJ, Gaylord EA, Griffin N, Sudiwala S, Cruz-Pacheco N, Emmerson E, Mohabbat S, Nathan S, Sinada H, et al.Tissue-Tek,Cat# 14-373-65 Dispase II,Gibco,Cat# 17105041 Cultrex 3D Culture Matrix Laminin I,R&D Systems,Cat# 3446-005-01 DMSO,Sigma-Aldrich,Cat # D2650 DMEM/F12,Thermo Fisher Scientific,Cat# 11320082 Penicillin-Streptomycin,Gibco,Cat#

(10) Quantification of spatial subclonal interactions enhancing the invasive phenotype of pediatric gliomaCell reportsAugust 30, 2022Tari H, Kessler K, Trahearn N, Werner B, Vinci M, Jones C, Sottoriva A.DNA fingerprinting. SU-DIPG-VI cells were maintained on laminin-coated flasks/plates at 10μg/ml (Bio-Techne, 3446-005-01), whereas HSJD-DIPG-007 cells were maintained as neurospheres. When the cells reached confluency, 90%

(11) Temporal proteomics during neurogenesis reveals large-scale proteome and organelle remodeling via selective autophagyMolecular cellDecember 16, 2021Ordureau A, Kraus F, Zhang J, An H, Park S, Ahfeldt T, Paulo JA, Harper JW.,Thermo Fisher Scientific,17502048 Neurotrophin-3,Peprotech,450-03 Brain-derived neurotrophic factor (BDNF),Peprotech,450-02 Cultrex 3D Culture Matrix Laminin I,R&D Systems,3446-005-01 B27,Thermo Fisher Scientific,17504001 Doxycycline,Sigma-Aldrich,D9891 Nunc Cell-Culture Treated 6-well,Thermo

(12) Impairment of Autophagic Flux Participates in the Antitumor Effects of TAT-Cx43266-283 in Glioblastoma Stem CellsCancers (Basel)August 24, 2021Sara G. Pelaz, Claudia Ollauri-Ibáñez, Concepción Lillo, Arantxa TaberneroCT, USA) (complete medium) under adherent conditions with laminin (R&D Systems, Minneapolis, MN, USA #3446-005-01). Cells were grown to confluence, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA,

(13) Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutantsCell stem cellMay 6, 2021Bressan RB, Southgate B, Ferguson KM, Blin C, Grant V, Alfazema N, Wills JC, Marques-Torrejon MA, Morrison GM, Ashmore J, et al.supplement,ThermoFisher,Cat. 17504044 N2 supplement,ThermoFisher,Cat. 17502048 Laminin-1,R&D systems,Cat. 3446-005-01 D-luciferin,Cayman Chemical,Cat. 14681-1 Benzonase nuclease,Sigma Aldrich,E1014 Recombinant Cas9

(14) Loss of Mediator complex subunit 13 (MED13) promotes resistance to alkylation through cyclin D1 upregulationNucleic acids researchFebruary 22, 2021Roliński M, Montaldo NP, Aksu ME, Fordyce Martin SL, Brambilla A, Kunath N, Johansen J, Erlandsen SE, Liabbak NB, Rian K, et al.plates. The plates were coated with 5 μg/ml PDL (A003E, Merck Millipore) overnight and 5 μg/ml Laminin (3446-005-01, R&D Systems) for 2 h. G144 cells were maintained in GBM medium [50% Dulbecco′s modified Eagle′s medium

(15) Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for VasculogenesisCell reportsAugust 11, 2020Rüdiger D, Kick K, Goychuk A, Vollmar AM, Frey E, Zahler S.

(16) Development of an in vitro intervertebral disc innervation model to screen neuroinhibitory biomaterialsJournal of Orthopaedic ResearchMay 1, 2020Sarah M. Romereim, Caleb A. Johnston, Adan L. Redwine, Rebecca A. Wachsprepared as described in the Base Hydrogel Composition section with the addition of 0.75 mg/ml laminin (344600501; R&D Systems) to enhance DRG cell attachment. Neuroinhibitory properties of CS biomaterials were initially

(17) Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic SignalingMolecular cellMarch 5, 2020Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW.(BDNF),Peprotech,Cat#450-02 Neurotrophin-3,Peprotech,Cat#450-03 Cultrex 3D Culture Matrix Laminin I,R&D Systems,Cat#3446-005-01 Y-27632 Dihydrochloride,Peprotech,Cat#1293823 Trysin,Promega,V511C Lys-C,Wako Chemicals,129-02541 Rapigest

(18) CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived NeuronsNeuronOctober 23, 2019Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, et al.(PeproTech; Cat. No. 450-03), 10ng/mL BDNF (PeproTech; Cat. No. 450-02), 1ug/mL Mouse Laminin (R&D Systems #3446-005-01), and optionally 2μg/mL doxycycline. Primary mouse astrocytes Primary mouse astrocytes were isolated

(19) Neutrophil extracellular traps produced during inflammation awaken dormant cancer cells in miceScience (New York, N.Y.)September 28, 2018Albrengues J, Shields MA, Ng D, Park CG, Ambrico A, Poindexter ME, Upadhyay P, Uyeminami DL, Pommier A, Küttner V, et al.culture-plates were coated with 50 μL of growth factor reduced matrigel (#356231, Corning) or mLN-111 (#3446–005-01, R & D Systems) and incubated at 37 °C for 30 min. The murine D2.0R cell line was used on matrigel or

(20) Ythdf2-mediated m⁶A mRNA clearance modulates neural development in miceGenome biologyMay 31, 2018Li M, Zhao X, Wang W, Shi H, Pan Q, Lu Z, Perez SP, Suganthan R, He C, Bjørås M, et al.dissociated cells were seeded into plates coated with PDL (Millipore, A-003-E) and laminin (R&D Systems, 3446–005-01). After 12-h culturing, cells were treated with 5 μg/ml actinomycin D (Sigma, A9415) for 2 and 4 h,

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Cultrex 3D Culture Matrix Laminin I from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Cultrex 3D Culture Matrix Laminin I from R&D Systems, a Bio-Techne Brand

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